Figure 4
Comparison of myoglobin spectra: charged manually (dotted) in the round cell holders using a 10 µm pathlength at ±7 mg ml−1 in CaF2 and in a microfluidic cell at 15 µm pathlength (dashes). The presented spectra were averaged over three raw spectra and their respective baselines subtracted, smoothed with step size of 5 (Savitzky Golay) followed by calibration to corresponding concentrations pathlengths and standardized to Δ∊ units using the mean residue weight of myoglobin (111.5 Da). For comparison a myoglobin spectrum from the reference data set SP175 (continuous line) is shown. |