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![[Figure 1]](hf5210fig1mag.jpg)
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Figure 1
Principle of using TEM-SRXRF to detect copper location in cells and tissues. The first step is to prepare the semithin sections of cells and tissues using a glutaraldehyde-fixed and resin-embedded approach, and to load the section onto a gold (Au) grid. TEM (solid bold line) and SRXRF (dashed bold lines) are then used to detect the cell or tissue ultrastructure and copper topography, respectively. For SRXRF, a monochromatic X-ray beam (blue) generated from a synchrotron radiation source was focused to a submicrometre spot on the sample section using a Fresnel zone plate (red). An energy-dispersive silicon drift detector (green) was used to collect the copper X-ray fluorescence spectrum. MAPS software was used to analyze the fluorescence data and to generate a copper image. The final SRXRF image (bottom right) represents copper topography in an aortic adventitial capillary vessel of a C57B/l mouse. The transmission electron micrograph of the same vessel (bottom left) shows an endothelial cell (marked EC), erythrocyte (marked E) and lumen (marked L). The image of a gold grid is derived from the Electron Microscopy Sciences website. Permission to use this image was granted. |
![[Figure 1]](hf5210fig1.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
