X-ray-induced photo-chemistry and X-ray absorption spectroscopy of biological samples

George, G.N.; Pickering, I.J.; Pushie, M.J.; Nienaber, K.; Hackett, M.J.; Ascone, I.; Hedman, B.; Hodgson, K.O.; Aitken, J.B.; Levina, A.; Glover, C.; Lay, P.A.

doi:10.1107/S090904951203943X

Journal of Synchrotron Radiation Journal of
Synchrotron Radiation

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 3
Fe K-edge XAS near-edge spectra showing X-ray-induced photo-reduction of superoxide reductase from Pyrococcus furiosus (data re-plotted from Clay et al., 2002

) showing (a) oxidized Fe(III) enzyme, (b) photo-reduced enzyme and (c) dithionite-reduced Fe(II) enzyme. The spectra of photo-reduced enzyme can be represented by a linear combination of Fe(III) and dithionite-reduced Fe(II) enzyme. The inset shows quantification of photo-reduction with time since start of irradiation using linear-combination fitting, indicating pseudo first-order kinetics (Clay et al., 2002

). Data were measured on SSRL BL7-3.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 19| Part 6| November 2012| Pages 875-886

https://doi.org/10.1107/S090904951203943X

Follow J. Synchrotron Rad.

Search IUCr Journals		doi		Advanced search
Author		volume	page