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Figure 1
SDS-PAGE (a, b) and western-blot (c) analyses of supernatant of ferritin-expressing E. coli lysates. Lanes (a) and (b) were stained with Coomassie Brilliant Blue and lane (c) with anti-HA-tag antibody. The HA-mFTH1 ferritin was observed primarily at 24 kDa, indicated by an arrow head, as well as at the top of the gel. Lanes (a) and (c): supernatant denatured at 298 K prior to SDS-PAGE; lane (b): supernatant denatured at 373 K. Molecular mass markers are indicated by labels.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 20| Part 4| May 2013| Pages 581-586
doi:10.1107/S0909049513008467
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