Figure 4
(a) Three-dimensional visualization of a cell pellet of ferritin-expressing recombinant with simple projection microtomograph. Capillary voxels surrounding the cell pellet were removed. Linear absorption coefficients are rendered from 8 cm−1 (white) to 50 cm−1 (black). Scale bar: 100 µm. (b) Three-dimensional visualization of cellular structures of ferritin-expressing recombinant visualized by Zernike phase contrast using a microtomograph equipped with a Fresnel zone plate. The recombinant cells were observed as curved columns (triangle) and ovals (arrows). This image was taken near the upper end of the cell pellet shown in (a). Scale bar: 2 µm. (c) Structures of mock recombinant stained with phosphotungstic acid. Scale bar: 2 µm. |