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Figure 3
(a) Bright-field for an intimin protein crystal generated in LCP with corresponding (b) trans-SHG and (c) X-ray raster summary overlay showing corrected Bragg-like reflection counts. (d) X-ray diffraction of the 5 µm-diameter area corresponding to the red circles in each image, with X-ray energy 12.0 keV, exposure time 1 s, photon flux 2.7 × 1010 photons s−1 (unattenuated beam), sample-to-detector distance of 300 mm, resulting in a maximum theoretical resolution of 2.25 Å. Scale bars are 50 µm. Cross-hairs were added to (a) and (b) to assist in orienting the field of view with respect to the diffraction raster images.

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