(a) Bright-field image of a membrane protein (human κ-opioid receptor complex) crystal in lipidic cubic phase and the corresponding (b) trans-SHG and (c) TPE-UVF, with (d) an X-ray raster summary overlay showing corrected Bragg-like reflection counts. (e) X-ray diffraction of the 5 µm-diameter area corresponding to the red circles in each image. X-ray energy: 12.0 keV; exposure time: 1 s; photon flux: 2.7 × 1010 photons s−1 (unattenuated beam); sample-to-detector distance: 300 mm; maximum theoretical resolution: 2.25 Å. Scale bars are 20 µm. Cross-hairs were added to (b) and (c) to assist in orienting the fields of view with respect to the bright-field and diffraction raster images.