Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline

Madden, J.T.; Toth, S.J.; Dettmar, C.M.; Newman, J.A.; Oglesbee, R.A.; Hedderich, H.G.; Everly, R.M.; Becker, M.; Ronau, J.A.; Buchanan, S.K.; Cherezov, V.; Morrow, M.E.; Xu, S.; Ferguson, D.; Makarov, O.; Das, C.; Fischetti, R.; Simpson, G.J.

doi:10.1107/S0909049513007942

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Figure 4
(a) Bright-field image of a membrane protein (human κ-opioid receptor complex) crystal in lipidic cubic phase and the corresponding (b) trans-SHG and (c) TPE-UVF, with (d) an X-ray raster summary overlay showing corrected Bragg-like reflection counts. (e) X-ray diffraction of the 5 µm-diameter area corresponding to the red circles in each image. X-ray energy: 12.0 keV; exposure time: 1 s; photon flux: 2.7 × 10¹⁰ photons s⁻¹ (unattenuated beam); sample-to-detector distance: 300 mm; maximum theoretical resolution: 2.25 Å. Scale bars are 20 µm. Cross-hairs were added to (b) and (c) to assist in orienting the fields of view with respect to the bright-field and diffraction raster images.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 20| Part 4| May 2013| Pages 531-540

doi:10.1107/S0909049513007942

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