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Figure 1
Elemental maps for K, P, Mn, Fe and Zn, and visible-light micrograph of a mixture of three cell types (red blood cells, algae, yeast) used for subsequent analysis. The quantified elemental content per pixel is shown in µg/cm2 in the intensity scale next to each map. The maps of particular elements obtained by X-ray fluorescence microscopy hint at the characteristics of the different cell types: Mn is prevalent in algae and Fe in red blood cells, while Zn and P are indicative of yeast cells. The visible-light micrograph was acquired at one focal plane and thus does not show all cells; slight distortions in relative cell positions between the X-ray fluorescence maps and the visible-light micrograph are due to differences in viewing angles.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 21| Part 3| May 2014| Pages 568-579
https://doi.org/10.1107/S1600577514001416
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