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![[Figure 5]](wa5055fig5mag.jpg)
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Figure 5
P, K, Mn, Fe, Zn content distributions of identified algae (green), yeast (blue) and red blood (red) cells. The light blue, pink and light green points indicate the estimated background (Bg) contents of the respective cell areas. The horizontal axis (log scale) shows net elemental content, and the points are distributed randomly within bounds in the vertical direction in order to provide separation. The magenta and black lines show, respectively, the initialized means from hand-drawn regions of interest, and the means of the actual cell populations in the characteristic elements. As expected, the cells tend to have high elemental content in their characteristic elements: red blood cells have high Fe content, and yeast cells contain significant amounts of P and Zn. Some elements can show negative content for some cells (the K channel in red blood cells, for example); this is because of the need to subtract substrate and background signals from the raw elemental content to calculate the actual elemental content for a given cell. In case the signal is close to zero, the statistical nature of the collected signal can lead to negative numbers when two nearly equal signals are subtracted. |
![[Figure 5]](wa5055fig5.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
