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![[Figure 5]](rv5012fig5mag.jpg)
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Figure 5
Dose response of residue M80 of cyt c. 10 µM of cyt c sample in 10 mM phosphate buffer, pH 7.0, was irradiated using the flow set-up at beamlines ALS 5.3.1, NSLS X28C and ALS 3.2.1. The deliverable dose on the sample was adjusted to be the same at 5.3.1 and X28C as determined by the standard Alexa assay. The fraction of unmodified peptide at a given exposure was calculated as the unmodified peak area divided by the sum of the unmodified and modified peak areas. The peak areas were calculated from the extracted ion chromatogram, and the site of modification was identified by MS/MS. The solid lines represent single exponential fits with rate constants k = 360 s−1, 330 s−1 and 14 s−1 for radiolysis carried out at 5.3.1 (black), X28C (red) and 3.2.1 (blue), respectively, with individual points representing the mean of three independent measurements with standard error. |
![[Figure 5]](rv5012fig5.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
