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Figure 2
Milestones in the progression of X-ray ptychography. (a) An X-ray demonstration of Wigner distribution deconvolution; shown are the amplitude (i) and phase (ii) of the transmission function of a set of latex spheres imaged with 3.1 nm radiation. [Reproduced from Chapman (1997BB7).] The scale bar represents 0.5 µm. This early work already included the simultaneous reconstruction of the illumination function, including aberration effects and partial coherence effects (Chapman, 1996BB6). (b) An early demonstration of X-ray ptychography, which was used as input to a tomographic reconstruction of a cylinder of murine femur. [Reproduced from Dierolf et al. (2010aBB14).]. (i) Cuts parallel and (ii) perpendicular to the axis of rotation. In both cases, the scale bar marks 5 µm. Such tomographic reconstructions are particularly reliable for quantitative density estimation. (iii) A histogram of the electron density of the entire tomogram and (iv) of two subvolumes of 1 µm3 each, demonstrating on such reduced spatial resolution a density specificity of ∼10−3 Å−3. (c) Demonstration of spectro-ptychography. Shown are both amplitude and phase images of a Balb/3T3 mouse fibroblast doped with cobalt ferrite (CoFe2O4) nanoparticles, whose contrast clearly vary upon scanning through the Fe LIII-edge. [Reprinted by permission from Macmillan Publishers Ltd: Nature Communications (Maiden et al., 2013BB51), copyright 2013.]

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