Figure 6
In order to align low-signal fluorescence projections onto a common rotation axis for tomography reconstruction, one can use other stronger signals. Shown here is the cross-correlation between two differential phase contrast images (one is shown at inset, upper right) and two Zn fluorescence images (one is shown at inset, upper left). Even though the simple differential phase contrast image is less easily interpretable than an image of the total projected phase, the cross-correlation peak is dramatically narrower, allowing much more precise alignment from this signal acquired simultaneously with the fluorescence data. The data shown here are for 3 ms dwell-time images of freeze-dried oocytes acquired at beamline 2-ID-E at the APS (Hong et al., 2014). |