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Figure 7
Self-absorption can give rise to errors and artifacts in fluorescence tomography (as well as incorrect ratios of elements) if not corrected. Shown here is the self-absorption calculated for fluorescence from a number of biologically interesting elements due to varying thicknesses of a 25% generic protein/75% water mix representing the cytosol of a typical cell (Luby-Phelps, 2000BB83).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 21| Part 5| August 2014| Pages 1031-1047
doi:10.1107/S160057751401621X
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