journal menu
![[Figure 2]](ig5011fig2mag.jpg)
|
|
Figure 2
Strongly diffracting insulin crystals with side length ratios of ∼4:4:1 are easily grown over a very large range between 2000 µm and 4 µm. A view of one 300 µm crystal is shown here. The flat rhomboid face of these crystals frequently rests against the supporting micro-mesh, orienting the crystallographic c axis nearly parallel to the X-ray beam. Although the 5, 10 and 20 µm specimens used in this study were too small to orient visually, the diffraction data confirms a significant bullet-shaped region of un-measurable nodes adjacent to the c axis (inset). Flat insulin crystals preferentially orient with the c axis orthogonal to the support (i.e. into the X-ray beam). 6° librations from 100+ reciprocal space wedges all around the c axis guarantee that the volume of measurable data (dotted line) retains constant thickness around the X-ray beam. However, a bullet-shaped region of reciprocal space remains occluded (shaded region). The situation is analogous to a Laue case with λmin ≃ 0.6 Å and λmax ≃ 2.0 Å (dashed line). Fortunately, the missing data were readily available by tilting the micro-mesh about omega prior to the grid scan. |
![[Figure 2]](ig5011fig2.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
