Solvent minimization induces preferential orientation and crystal clustering in serial micro-crystallography on micro-meshes, in situ plates and on a movable crystal conveyor belt

Soares, A.S.; Mullen, J.D.; Parekh, R.M.; McCarthy, G.S.; Roessler, C.G.; Jackimowicz, R.; Skinner, J.M.; Orville, A.M.; Allaire, M.; Sweet, R.M.

doi:10.1107/S1600577514017731

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Figure 4
Solvent minimization. The signal-to-noise ratio [I/σ(I)] is plotted for the same thermolysin crystal at room temperature before solvent minimization (a), at room temperature after solvent minimization (b) and at 100 K (c). The room-temperature data sets consisted of 0.1° rotations with a duration of 0.2 s, with the X-ray beam attenuated by 97%. The 100 K data set consisted of 2.0° rotations with a duration of 2.0 s, using the full X-ray beam. The inset shows the 8 $[\overline {23}]$ $[\overline {20}]$ reflection after solvent minimization. The background integration box for this reflection had exactly zero observed counts for 60% of the pixels (so that the readout value was equal to the Pilatus 6M baseline of 1).

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 21| Part 6| October 2014| Pages 1231-1239

doi:10.1107/S1600577514017731

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