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![[Figure 5]](ig5011fig5mag.jpg)
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Figure 5
These graphs show completeness of the data from porcine insulin (percent) against the number of 6° data wedges that were merged together from crystals on micro-meshes (a), on in situ plates (b) and on a movable conveyor belt (c). Two plots are shown in each panel. The lower plot shows completeness as 55 × 6° data wedges are merged with the flat surface of the data-collection media normal to the X-ray beam, followed by 33 × 6° data wedges with the flat surface offset to the X-ray beam by 30° (plates) or 45° (micro-meshes and conveyor belt). In the upper plot the offset data wedges are merged by themselves. An asymptotic best fit shows that, without the offset, the completeness of the data approaches a limit value of less than 100%. In contrast, when offset data are included (either alone or in combination with the `flat' data), 100% completeness is achieved. The data were smoothed by merging the 6° wedges in 100 random permutations, and averaging the results. The inset shows a similar calculation for 6° wedges of data from 55 lysozyme crystals (panel α). The lysozyme crystals do not appear to preferentially orient their crystallographic axes relative to the micro-mesh surface. The completeness of the merged data file rapidly approaches 100% regardless of whether the micro-mesh is presented normal to the X-ray beam or at an angle. The same results were observed with lysozyme crystals in Grainer in situ plates and on the conveyor belt (data not shown). |
![[Figure 5]](ig5011fig5.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
