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Figure 6
Careful minimization of mother liquor was an indispensable step to observe adequate signal-to-noise in the diffraction pattern. One undesired side effect is that solvent minimization induces crystals to aggregate into tight clusters of crystals that yield overlapping diffraction patterns. The 5 µm insulin crystals shown here diffracted to 2.3 Å, but no structure was forthcoming because of persistent overlap problems. Realistic molecular-replacement structures were readily obtained from 10 µm crystals and from 20 µm crystals.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 21| Part 6| October 2014| Pages 1231-1239
doi:10.1107/S1600577514017731
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