Figure 1
Experimental setup. An OVA allergen-induced experimental allergic airways disease model was used, consisting of two immunization steps (i.p. injection of 10 µg OVA) on days 0 and 21 and two challenging steps (i.n. application of 100 µg OVA) on days 28 and 29. Prior to their instillation, MΦ were labelled with Micropaque and DiD for 24 and 4 h, respectively. On day 32, 6 × 106 barium- and DiD-labelled MΦ were instilled i.t. into asthmatic and control mice. Mice were euthanized on day 33 and prepared for fXPCT analysis. |