Functionalized synchrotron in-line phase-contrast computed tomography: a novel approach for simultaneous quantification of structural alterations and localization of barium-labelled alveolar macrophages within mouse lung samples

Dullin, C.; dal Monego, S.; Larsson, E.; Mohammadi, S.; Krenkel, M.; Garrovo, C.; Biffi, S.; Lorenzon, A.; Markus, A.; Napp, J.; Salditt, T.; Accardo, A.; Alves, F.; Tromba, G.

doi:10.1107/S1600577514021730

Figure 3
Volume rendering of the fXPCT results and visualization of the MΦ localization. Volume renderings of four 3D reconstructions of phase-retrieved fXPCT data sets are shown. Each column represents one lung sample of an asthmatic mouse (AA), a healthy mouse without instilled MΦ as negative control (Blk), and two healthy controls (CN1, CN2). Bone is displayed in grey, soft-tissue in red and barium in yellow (air is not depicted). (a) Illustration of the entire field of view: more barium and an increased soft-tissue density is shown in AA. (b) VOIs (their origin is indicated by white squares in row 1). (c) The same VOIs are shown but with higher transparency to allow visualization of the sites where barium-filled MΦ cluster in more detail. Note that in the VOIs of the AA lung a higher barium content is visible compared with the results obtained in lungs of the negative control Blk and the healthy controls CN1 and CN2. Additionally, the VOIs of the AA sample show a higher soft-tissue content in comparison with the VOIs of all controls, reflecting a reduced pulmonary volume and increased soft-tissue content in the asthmatic lung tissue.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 22| Part 1| January 2015| Pages 143-155

https://doi.org/10.1107/S1600577514021730

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