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![[Figure 5]](mo5097fig5mag.jpg)
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Figure 5
Validation of the fXPCT results by fluorescence microscopy, HR microCT and histology. (a, b) Fluorescence microscopy of lung sections of (a) an asthmatic (AA) and (b) a control mouse (CN). Nuclei were stained with DAPI (blue) and MΦ were immuno-stained with an anti-CD68 antibody (green). DiD-labelled MΦ are shown in red, whereas instilled MΦ appear yellow due to the double staining with CD68 and DiD. Endogenous MΦ are shown in green and can be seen in alveoli of both AA and CN lungs, but seem to form clusters in AA (a and b). Instilled DiD-labelled MΦ are also visible in both AA and CN lung samples (white arrows). In contrast to the CN, the AA sample shows high cellular density areas (white rectangle and detailed view), where several CD68 positive cells and clusters of instilled MΦ can be observed. Unlike the AA sample, the CN does not show these areas, which may explain the increased soft-tissue ratio and the airway obstruction found in the fXPCT results. (c, d) Representative cross sections of HR microCT for AA and CN vibratome lung section. An increased wall thickness (white arrow head) for AA (c) and the location of the labelled MΦ (black dots) can be clearly depicted. MΦ can be seen in both CN and AA, (c) shows an area with higher cell density and accumulation of MΦ (white rectangle). (e and f) PAS-stained lung sections of an asthmatic (AA) and a control mouse (CN). Increased wall thickness in asthmatic lung tissue (black arrow head) is depicted. Red dots are exclusively visible in asthmatic lungs and depict mucus production. |
![[Figure 5]](mo5097fig5.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
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