Figure 2
The effects of beam attenuation. SAXS data (5 × 50 ms exposures) recorded from static 4.4 mg ml−1 lysozyme samples collected at: (a) 5.1 × 1012 photons s−1, (b) 7.3 × 1011 photons s−1, (c) 1.8 × 1011 photons s−1. The critical dose, in kGy, for the lysozyme samples at each level of attenuation is reported on each plot. (d) The initial rates of damage (black diamonds) are compared with the initial rate of damage at full beam flux with sample flow enabled (white circle). Initial rates were estimated from static samples exposed to approximately the same dose per frame by adjusting the exposure time at each level of attenuation (50 ms, 0.32 s and 1.41 s, for no, medium and high attenuation, respectively). |