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Figure 4
Radiation susceptibilities of different proteins and the effect of solution additives. (a) Histogram comparing the initial rates of radiation damage calculated across 5 × 30 ms exposure frames for GI (10 mg ml−1), BSA (11 mg ml−1), cytochrome-C (Cyto, 10 mg ml−1), lysozyme (Lyso, 8.8 mg ml−1) and RNAse (10 mg ml−1) with and without solution additives present (Asc, ascorbate, 1 mM; DTT, dithiothreitol, 1 mM; glycerol, 5% v/v; GI/glycerol and Cyto/glycerol measurements are not included). Estimates of the critical dose (kGy) are reported (for further details refer to Table S4 of the supporting information). (b) The effect of 5% v/v glycerol on the solvent-corrected SAXS scattering intensities of BSA in solution as a consequence of reducing X-ray contrast.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 22| Part 2| February 2015| Pages 273-279
doi:10.1107/S1600577515000375
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