Figure 5
Radiation damage to GLU O atoms. Top: lysozyme (left, four GLU O atoms) was subjected to a 2 MGy X-ray burn, ribonuclease A (right, ten GLU O atoms) to a 4 MGy burn. The increase in B-factors is barely visible and within a margin of error for lysozyme. The increase for ribonuclease A is more visible. The coloured box of the boxplot indicates the interquartile range. Bottom: BDamage shows a significant change in the GLU O atoms, indicating that this part of the protein degrades more rapidly than other comparable sites. The difference in BDamage is larger for ribonuclease A (right), consistent with its exposure to a significantly higher dose. |