Figure 4
Angular dependence of reflectivity and Zn fluorescence yield from the protein films of alkaline phosphatase immobilized on the monolayer of cellulose acetopivalinate; samples N2 (SI-CAP)+AP (a) and sample N6 (SI-CAP)+AP[Pb] (b). Curve 1: the calculated angular dependence of the fluorescence yield from the film, in which zinc ions are distributed in a layer with a thickness of 10 Å at the bottom of the protein monolayer; curve 2: the calculated angular dependence of the fluorescence yield from the film, in which zinc ions are distributed over the whole thickness of the protein layer (50 Å). |