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![[Figure 3]](vv5105fig3mag.jpg)
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Figure 3
Correlative microscopy of PtK2 infected cells with vaccinia virus. In vivo light microscopy (a) and epifluorescence images (b) of the cells growing on a holey carbon grid. In (b), DAPI (in blue) labels the DNA, WGA (in red) labels membranes of the cell and GFP (in green) is expressed only by the infected cells. (c) 2D X-ray mosaic composition (images stitching) of the same area of the grid shown in (a) and (b). The inset shows a cryo bright-field light microscopy image with the on-line light microscope of the TXM in which the two cells of (a) and (b) are clearly visible. The arrows from (a) to (c) point to the position of the same infected cell. (d) −65°, 0° and 65° TXM projection images of the selected cell at higher magnification. (e) Virtual slice of the reconstructed soft X-ray cryo-tomogram in the xy plane. N marks the position of the cell nucleus. (f) Segmented volume in the xy plane of the 3D tomographic reconstruction. The nucleus in blue is surrounded by two different viral forms, mature and immature, inside the cell in red and yellow, respectively. |
![[Figure 3]](vv5105fig3.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
