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Figure 4
(a) Dark-field overview scan of a frozen hydrated Dictyostelium discoideum cell with 1 µm step size and 0.5 s dwell time (continuous scan). Scale bar: 10 µm. (b) Exemplary far-field patterns recorded with 10 s accumulation time in an array of 6 × 6 points in the region indicated by the white square in (a). While the diffraction patterns are radially symmetric in the centre, anisotropic distributions are found at the periphery of the cell, which have been attributed to actin fibre bundles. (c) Horizontal KB beam profile without and with soft-edge cleaning apertures, as used to obtain the data in (a, b, d, f). Note the steep decay of the KB profile over seven orders of magnitude! Also shown is the slight suppression of background resulting from the BS in front of the flight tube. (d) Example of an anisotropic diffraction pattern at the location indicated by the cross in (a). Scale bar: 0.1 nm−1. (e) Simulated diffraction profile of two filaments crossing at an angle varied to match the data in (d). Note that the extension of strong diffraction is orthogonal to the fibre bundle axis. Scale bar: 0.1 nm−1.

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