Figure 1
Mouse restraint device. (a) Schematic of the mouse restraint illustrating the anaesthetic supply cone (i) which directly connects to an anaesthetic supply and recovery hose. The restraint cone (ii) freely rotates under the anaesthetic supply cone and focuses the flow of anaesthesia onto the mouse's nose. A clear acrylic tube (iii) contains the mouse and allows for visual monitoring of respiration rate. The front half of the restraint (iv) was designed to house physiological infrared sensors. The back half of the restraint (v) was designed with an upper portion for the mouse and a lower portion for a temperature probe. The restraint base (vi) locks together both pieces of the restraint and magnetically attaches to the beamline imaging stage. (b) Photograph of fully assembled three-dimensional-printed mouse restraint in black polylactic acid. (c) Closed-circuit camera photograph showing the setup at the CLS BMIT-BM beamline. The anaesthetic supply (clear tube) and recovery hose (blue tube) are shown fixed above the rotating restraint. The Kent Scientific PhysioSuite™ monitoring module with wires connected to the mouse restraint device to measure HR, SpO2, RR and core body temperature sits to the left of the rotating imaging stage. |