Figure 2
Absorption spectral changes during X-ray irradiation of the heme in an NO-bound ferric P450nor crystal at 100 K. The spectra with 60 ms exposure were obtained every 1 s after initiation of X-ray irradiation. Five spectra with X-ray irradiation times of 1 s (red), 10 s (orange), 100 s (green), 300 s (blue) and 900 s (purple) are shown. The temporal change in the absorbance at 561 nm is shown in the inset. The temporal change was fitted with a double exponential function with time constants of 16 and 400 s. |