Figure 3
Visible absorption spectra of a ferric P450nor crystal containing caged NO at 100 K before UV irradiation (red), after UV irradiation (blue) and after annealing for 3 s (green). The spectrum of an NO-bound ferric P450nor crystal prepared with NO gas is shown in black as a reference. A linear background subtraction was performed to overlay the spectra for comparison. Each spectrum shown is the average of 500 data, each obtained with 60 ms exposure. |