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Figure 2
Synchrotron infrared microscopy analyses. (a) Reflection spectra before the FEL irradiation. Solid line: keratin monomer; dotted line: keratin aggregate; red dotted line: the difference spectrum. The scale for the difference spectrum is shown on the right-hand side of the figure. Arrows indicate FEL wavelengths for irradiation. (b) De-convolution spectra around the amide I band. (c)–(f) Reflection spectra after FEL irradiation at several wavelengths (solid lines). Dotted line: keratin aggregate; red dotted line: the difference spectrum, (c) 6.06 µm, (d) 6.51 µm, (e) 8.06 µm, (f) 5.60 µm. (g) Secondary structure analyses of the keratin monomer, keratin aggregate, keratin aggregate after the FEL irradiation at 5.60, 6.06, 6.51 and 8.06 µm. Black bar: α-helix; white bar: β-sheet; dark gray: β-turn; light gray: non-ordered structure.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 23| Part 1| January 2016| Pages 152-157
doi:10.1107/S1600577515020731
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