Figure 6
Result of the first stage of estimating the most probable overall support shape of a chloroplast from the diffraction pattern in Fig. 3(b). (a) A projection of 1000 electron density maps in the 2500-dimensional space onto the plane spanned by the first two PCs determined in PCA. The positions of maps are indicated by symbols colored according to the classes from the k-means clustering. (b) The distribution in panel (a) expressed as the frequency. (c) The averaged electron density map and averaged support shape of each class. The scale bars indicate 500 nm. The values of parameters for each class are compiled in Table 3. (d) A magnified view of the averaged electron density map of the selected class 6. (e) A PRTF curve calculated from 137 electron density maps belonging to class 6. The dotted lines are used to estimate the effective resolution of the averaged map. |