Figure 2
Comparison of Alcian blue staining in three-dimensional printed cell-impregnated constructs, showing secretion of sulfated GAGs at different time points. Panels A–D demonstrate progressive secretion of sulfated GAGs, and E–H are high-magnification views of the regions of interest highlighted in the red boxes of panels A–D. Panels I–L are cross-section images through the centre of the hybrid constructs. Panel M represents the quantitative analysis of Alcian blue stained area in the hybrid constructs at days zero, 14, 28 and 42 showing statistically significant difference in secretion of GAGs at the different time points. |