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Figure 3
Comparison of Col2 immunostaining and DNA labelling in three-dimensional printed cell-impregnated constructs at different time points. Panels A–D and E–H show progressive secretion of Col2 and corresponding DAPI staining, respectively. Panels I–L are cross-section images through the centre of the hybrid constructs. Panels M and N are high-magnification views of the upper region of interest outlined in red boxes of panels D and H, whereas panels O and P are high-magnification views of the lower region of interest outlined in red boxes of panels D and H. Panel Q represents quantitation of Col2 immunostained area in the hybrid constructs, showing statistically significant differences in Col2 secretion at different time points.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 23| Part 3| May 2016| Pages 802-812
doi:10.1107/S1600577516002344
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