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Figure 8
Diffraction patterns from flash-cooled cyanobacteria specimens with the frequency distribution regarding the total diffraction intensities and maximum resolutions. The total intensity of each diffraction pattern was calculated over the area displayed. The maximum resolution is defined as the highest-resolution shell including at least three detector pixels with more than four photons (Sekiguchi et al., 2014bBB54). We did not analyze diffraction patterns with less than three detector pixels, which count more than four photons. The data shown are those for cyanobacteria surrounded by an appropriate amount of buffer solution (a), semi-dried (b) and heavily wet (c). The electron density maps retrieved from the diffraction patterns in Figs. 8(a) and 8(b)[link] are shown in the inset. We applied the ASURA protocol to these diffraction patterns up to a resolution of 50 nm at edge. The statistics of the retrieved electron density maps are listed in Table 1[link].

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