Specimen preparation for cryogenic coherent X-ray diffraction imaging of biological cells and cellular organelles by using the X-ray free-electron laser at SACLA

Kobayashi, A.; Sekiguchi, Y.; Oroguchi, T.; Okajima, K.; Fukuda, A.; Oide, M.; Yamamoto, M.; Nakasako, M.

doi:10.1107/S1600577516007736

Journal of Synchrotron Radiation Journal of
Synchrotron Radiation

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Figure 9
Two representative examples of diffraction patterns and the most probable electron density maps of frozen-hydrated single cyanobacterial cells. We applied the ASURA protocol to the diffraction patterns up to a resolution of 50 nm at edge. The statistics of the diffraction patterns and phase-retrieved projection electron density maps are summarized in Table 1

. The effective resolutions of the density maps are estimated by using the PRTF. Size distribution of cyanobacterial cells for 63 retrieved projection electron density maps (c), and that measured by DLS (d). The inset shows an image of a cyanobacterial cell taken using an IX71 optical microscope.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 23| Part 4| July 2016| Pages 975-989

https://doi.org/10.1107/S1600577516007736

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