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Figure 1
Multimodal images of mCherry crystals acquired at cryogenic temperature are shown. Images in the top row were acquired simultaneously using 532 nm incident light for one-photon-excited fluorescence in (a), two-photon-excited ultraviolet fluorescence in (b) and laser transmittance bright field of the residual 1064 nm light in (c). Images in the bottom row were acquired simultaneously using 1064 nm incident light for second-harmonic generation in (d), two-photon-excited visible fluorescence in (e) and laser transmittance bright field in (f). All images were acquired with a 0.25 µs pixel dwell time with 512 frames averaged together.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 23| Part 4| July 2016| Pages 959-965
doi:10.1107/S1600577516005919
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