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Figure 4
Detection of the site of bound water interaction by temperature-dependent XF-MS. The colored surfaces on the X-ray crystal structures of cyt c (PDB 1HRC) (a) and ubiquitin (PDB 1UBQ) (b) indicate the side-chain residues that are consistently modified at room temperature and/or frozen conditions. Blue indicates the residues that show a 13- to 200-fold decrease in modification upon freezing; violet indicates a 3- to 10-fold decrease; and red indicates minimal to no change (<2-fold) in the modification rate when the sample is frozen as compared with room temperature. In cyt c the residue Y67, which also showed minimal to no change, is completely buried inside the heme cavity and not visible in these orientations. Reproduced from Gupta et al. (2012BB36).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 23| Part 5| September 2016| Pages 1056-1069
https://doi.org/10.1107/S1600577516009024
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