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Figure 2
(a) Categorization of arterial branching order. After the main vessel (A0) branch, the number of vessels increases with each successive branch. (b) Setting of the region of interest (ROI) in a temporally subtracted microgram at A0 for diameter measurement, and depiction of the moving averaged intensity profile over the first 300 frames (upper right panel) of the subtracted image, and the time intensity curve of the contrast media in the ROI (line with circles) alongside the intensity differential plot (pale gray line) of the time intensity curve (lower panel). The shaded region indicates the start and finish of the frames used for determination of the gradient of the slope (SG), which was used as the index of arterial blood flow at each branch point. The intensity average of frames 1–40 is indicated as the background zero intensity under the time intensity curve (horizontal black dashed lines). Mean transition time (MTT) was calculated as the duration that the intensity was sustained at half the maximum intensity gray level as a second estimate of blood flow.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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