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![[Figure 5]](fv5080fig5mag.jpg)
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Figure 5
Challenges in expert hit recognition. (a) The large unit cell of photosystem II presents a clear, densely spaced lattice with CSPAD detection. (b, c) The smaller unit cell and high crystalline order of thermolysin generate a more difficult scenario. Some images (b) show an easily recognized lattice, while others (c) require careful inspection. Several Bragg spots in (c) have only a single pixel square area, and can only be recognized by contrast optimization, blink comparison against neighboring images and evaluation of the spot density, which is greatest at low scattering angle. (d, e, f) Rayonix images with a large point spread function often display easily recognized spots; however, weak spots are harder to recognize because they lack contrast with the background. Arrows indicate (b) a diffraction spike due to the liquid jet sample-delivery system, (c) thermolysin Bragg spots 2–3 pixels in square area, that are oriented radially with respect to the direct beam, (d) a hydrogenase spot with area nearly 400 pixels in square area, due to large spot intensity and point spread function, and (f) photosystem II Bragg spots with very low contrast against the background. |
![[Figure 5]](fv5080fig5.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
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