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Figure 2
(a) Dose dependent FTIR absorbance spectra of a region of kidney tissues with different fixation protocols: green (bottom), formalin; blue (middle), glutaraldehyde; violet (top), Karnovsky. Radiation doses go from continuous line Rad0 to progressively dotted line Rad4. Spectral groups are displayed with an offset for clarity (in arbitary units). Grey lines refer to components from the ultralene (2960, 2920, 2870, 2839, 2722, 1457, 1378, 1359, 1326, 1303, 1254, 1168, 1103, 1043, 996, 973 cm−1), orange lines mark components derived from paraffin on ultralene (3071, 2851, 1710, 1640 cm−1) and fuchsia lines mark the components from the tissues (3295, 1652, 1536 cm−1). (bc) Second derivatives of the studied tissues with the different fixation protocols, liver and kidneys, respectively. Grey lines refer to the bands at 1713, 1662, 1655 and 1643 cm−1 (from left to right).

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