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Figure 1
Comparison of SRµCT-based virtual histology of mouse lungs using three different staining protocols. Representative SRµCT scans of mouse lungs embedded in paraffin (voxel size = 2.6 µm) and stained with three different protocols (upper row): (a) PTA staining, (b) I2 staining and (c) chrome alum staining. The detailed views (lower row) of the regions of interest (black squares) depicted in the overviews (upper row) show that all staining procedures allow a precise delineation of the lung parenchyma. In addition, scans of both PTA (a) and I2 stained lungs (b) showed an increased contrast of bronchi (b) and vessel walls (v). In (c), only the blood within the vessels is highlighted (v). Note that in the I2 stained sample, the blood is strongly stained allowing the differentiation between the vessel tree and the bronchial system of the respiratory tract.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 25| Part 4| July 2018| Pages 1153-1161
https://doi.org/10.1107/S1600577518005489
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