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Figure 4
Comparison of SRµCT scans of an unstained and a PTA stained mouse brain embedded in paraffin: SRµCT scans of paraffin-embedded mouse brains, unstained (a) and PTA stained (b), and acquired with the same parameters, show a comparable area within the cerebellum. In (a) the structure of the cerebellum of the unstained brain is clearly distinguishable, especially in the detailed view (insert), demonstrating that the brain can be studied at cellular resolution. The different anatomical structures of the cerebellum of the PTA stained brain are also visualized in (b), but only at a limited level of detail in comparison with (a). (c) The radially averaged power spectra of the unstained brain data (red) show less content of low-frequency details and a higher content of high-frequency details (black arrow heads) than in the power spectrum of the PTA stained brain (black curve). In addition, the noise level is reduced in the unstained tissue as shown by the drop of the red curve for the smallest wavelengths (white arrow head).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 25| Part 4| July 2018| Pages 1153-1161
https://doi.org/10.1107/S1600577518005489
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