Figure 6
Holographic projections and iterative reconstructions from barium-stained macrophage during rotation in an optical trap. (a) Holographic recordings of the rotating cell. Strong fringes appear due to the barium sulfate stain. (b) Barium sulfate grains become clearly resolved upon iterative reconstruction. Here, 500 iterations using the RAAR scheme have been used for a good reconstruction. Higher gray values in the center of the cell seem to depict the cell body. The inset shows single barium sulfate particles. Laser trapping power: 100 mW. Scale bar: 5 µm. Scale bar of inset: 500 nm. |