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Figure 11
Sketch of the best-focus PSFs' light intensity distribution in the XY plane. (a1)–(d1) PSFs with pseudo-color. (a2)–(d2) PSFs with three-dimensional maps. (a1)–(a2) 10× objective and 4.6 µm-thick GGG:Tb. (b1)–(b2) 10× objective and 20 µm-thick GGG:Tb. (c1)–(c2) 40× objective and 4.6 µm-thick GGG:Tb. (d1)–(d2) 40× objective and 20 µm-thick GGG:Tb. In (a1)–(b1) and (a2)–(b2), the pixel size in object space is about 0.3939 µm, and the pixel size in (c1)–(d1) and (c2)–(d2) is about 0.0985 µm. In the figure, when the objective is the same, the thicker the SCF, the more diffuse the best-focus PSF becomes.

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