Figure 8
The changes of the detector's PSFs in the XZ or YZ section during the focusing process. The conditions corresponding to (a) are 10× objective, 4.6 µm-thick GGG:Tb; (b) are 10× objective, 20 µm-thick GGG:Tb; (c) are 10× objective, 4.6 µm-thick GGG:Tb; (d) are 10× objective, 20 µm-thick GGG:Tb. In each case, the position of the best-focus PSF is marked with a purple dashed line. |