Figure 9
(a) Ideal image in object space with a spatial frequency of about 127 line-pairs mm−1 and a contrast of 90.48% for the 10× objective. (b) Ideal image in object space with a spatial frequency of about 508 line-pairs mm−1 and a contrast of 90.48% for the 40× objective. (g, h) Diagrams of the contrast in the blurred images after images (a) and (b) were convolved with the PSFs from Fig. 8. For each case there is a PSF that maximizes the contrast in the blurred images. We call this corresponding PSF the detector's best-focus PSF. The positions of the best focus were marked in the curves. (c)–(f) Blurred images which are simulated at the positions marked in (h) when the 40× objective was focused on a 20 µm-thick GGG:Tb. We can see that the blurred image in the best focus position has the best image quality. |