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Figure 3
(a) Schematic illustration showing the procedure to set a specimen particle on a PLL-coated silicon nitride membrane. (b) Photograph of the flash-cooling device with a telescope for checking flash-cooled specimens. A specimen disk is plunged into liquid ethane kept at 85 K by using a film heater to prevent solidification. A telescope with a long working distance was placed outside the liquid-nitro­gen bath to monitor the flash-cooled specimen particle through a slide glass fixed to a window of the liquid-nitro­gen bath. The upper right panel shows an arrangement of devices inside the liquid-nitro­gen bath. Dry air is supplied to the surface of the slide glasses to prevent dew formation (lower right panel). (c) LM image of a specimen on a silicon nitride membrane (left panel) and a magnified image (middle) before flash-cooling. The right-hand panel shows a telescope view of the specimen after flash-cooling. The specimen particle is indicated by a red arrow in each panel. In this case, the remained buffer near the specimen particle (a cloud-like object at the upper right) is a good landmark to identify the position of the particle in all images.

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ISSN: 1600-5775
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