Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation

Kobayashi, A.; Takayama, Y.; Okajima, K.; Oide, M.; Yamamoto, T.; Sekiguchi, Y.; Oroguchi, T.; Nakasako, M.; Kohmura, Y.; Yamamoto, M.; Hoshi, T.; Torizuka, Y.

doi:10.1107/S1600577518012687

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Figure 7
Projection and 3D electron density maps of C. merolae. (a) Projection electron density maps retrieved from the diffraction patterns in panel (a) of Fig. 6

at a resolution of 136 nm. The scale bar is 5 µm. The variation of T_ij (b) and crystallographic R-factor (c) values of the most probable PR maps from diffraction patterns are plotted against the rotation angle. Four views of 3D electron density maps of C. merolae reconstructed by the back-projection method (d) and 3D PR method (e). The maps are contoured to demonstrate the cell envelope and high-density regions. The model is illustrated by using the UCSF Chimera suite (Pettersen et al., 2004

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 25| Part 6| November 2018| Pages 1803-1818

https://doi.org/10.1107/S1600577518012687

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