Diffraction apparatus and procedure in tomography X-ray diffraction imaging for biological cells at cryogenic temperature using synchrotron X-ray radiation

Kobayashi, A.; Takayama, Y.; Okajima, K.; Oide, M.; Yamamoto, T.; Sekiguchi, Y.; Oroguchi, T.; Nakasako, M.; Kohmura, Y.; Yamamoto, M.; Hoshi, T.; Torizuka, Y.

doi:10.1107/S1600577518012687

Figure 9
(a) The left panel shows a diffraction pattern of a C. merolae cell in the G/M phase recorded at an exposure time of 2760 s. The dotted circles display the resolution in reciprocal space. The area indicated by the dashed box is magnified in the upper-right panel. The lower-right panel is a projection electron density map retrieved from the diffraction pattern in the left panel at a resolution of 34.1 nm. (b) Representative diffraction patterns at an edge region of the detector during the accumulation of 552 frames of 5 s exposure. (c) The variation of the maximum resolution of diffraction pattern plotted against the exposure time. The dashed line indicates a linear correlation between the maximum resolution in reciprocal space and the logarithm of the exposure time. (d) Plot showing the number of exposures to achieve a spatial resolution in real space. (e) Estimated radiation dose in a tomography experiment to collect diffraction patterns necessary for the 3D reconstruction of a map at a desired resolution in real space (red circles). The blue line is the resolution-dependence of the maximum tolerable radiation dose reported in the literature (Howells et al., 2009

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 25| Part 6| November 2018| Pages 1803-1818

https://doi.org/10.1107/S1600577518012687

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