A comparison of parametric and integrative approaches for X-ray fluorescence analysis applied to a Stroke model

Crawford, A.M.; Sylvain, N.J.; Hou, H.; Hackett, M.J.; Pushie, M.J.; Pickering, I.J.; George, G.N.; Kelly, M.E.

doi:10.1107/S1600577518010895

Figure 5
Xspress 3 data analyses and comparisons: (a)–(g) are from an adjacent section to that of Fig. 3

and were collected at BL 14-3; (h)–(k) are based on a high-resolution image of that same section collected at BL 2-3. (a) and (b) Binned K and Ca images along with their fitted counterparts (c) and (d) from an adjacent section to the image in Fig. 3

. (e) and (f) The per-pixel correlations of binned Ca as a function of fitted Ca; the dashed black line indicates a ratio of 1:1. The partitioned regions have been restructured into the image in (f). A third region was partitioned by using the morphological structure in (c), demarcated by the arrows, as the outline for a second boundary. These regions have been labeled 1, 2 and 3 and are overlaid on (a)–(d) to represent the healthy tissue, peri-infarct zone and infarct, respectively. (g) A bar plot showing the apparent [Ca] in healthy tissue, peri-infarct zone and infarct from binned and fitted analyses for the tissue sections represented by (f). (j) and (k) A close up high-resolution scan of Ca within the infarct (rectangle region in f) analyzed using binning (j) and fitting (k). (h) Correlation plot of fitted versus binned Ca from (j) and (k). (i) Reconstructed image from the separated cluster from (h). Scale bars: (a)–(d), 500 µm; (j) and (k), 20 µm. Intensity bars are in units of µg cm⁻².

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 25| Part 6| November 2018| Pages 1780-1789

https://doi.org/10.1107/S1600577518010895

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